Latest topics
» Make America Great Again - Legalizing Cannabis Will Do That
Yesterday at 7:38 pm by PurpleSkyz

» DARK JOURNALIST X-SERIES XXII: SUPRA ET ULTRA X-TECH UFO ENERGY SECRET!
Yesterday at 7:30 pm by PurpleSkyz

» The Story that CNN Would Never Air — Collapse of the Clinton
Yesterday at 7:09 pm by PurpleSkyz

» China’s vaccine scandal explodes as one million doses now found to be maiming Chinese children
Yesterday at 7:05 pm by PurpleSkyz

» #QAnon - Why Are All These Men Wearing Red Shoes??
Yesterday at 6:54 pm by PurpleSkyz

» *Deep 6.5 EQ Indonesia*220,000 Homeless India Flooding*Iranian Sleeper Cells*Italy Earthquake Swarm*
Yesterday at 3:44 pm by PurpleSkyz

» Aretha Franklin Has passed away-
Yesterday at 3:34 pm by PurpleSkyz

» The GoldFish Report No. 261 Country Roads: Legal Bootstrapping: How State’s Claim Title of a Body
Yesterday at 3:30 pm by PurpleSkyz

» POOFness for AUG 15: MIDWEEK BY ZAP 8-15-18
Yesterday at 2:44 pm by 4-truth

» Another April LaJune Client Speaks Out!
Yesterday at 1:43 pm by PurpleSkyz

» #QAnon - We Are in Control plus more
Yesterday at 11:29 am by PurpleSkyz

» The World Behind the Curtain: The Story Of Your Enslavement
Yesterday at 11:25 am by PurpleSkyz

» Kp Message 8-17-18… “What’s going on with the current pathway”
Yesterday at 11:09 am by PurpleSkyz

» “Facebook Threatened By New Social Network That Will RESTORE Freedom To the Internet”
Yesterday at 11:08 am by PurpleSkyz

» Cindy Kay Currier - I Am Crazy
Yesterday at 11:04 am by PurpleSkyz

» Primaries of foundations come to the fore
Yesterday at 10:18 am by PurpleSkyz

» UFO Shot Down Over Turkey Explodes Over City
Yesterday at 10:03 am by PurpleSkyz

» UFO News ~ Three Green Orbs Recorded Over Lake In British Colombia, Canada plus MORE
Yesterday at 10:00 am by PurpleSkyz

» Nibiru News ~ NIBIRU SYSTEM - THEY CANNOT HIDE IT plus MORE
Yesterday at 9:57 am by PurpleSkyz

» *2 Rivers Disappear France*6.6 EQ Hits Off Coast Of Japan*Italy Rocked 5.3 EQ*Tsunami Warning UK*
Yesterday at 9:49 am by PurpleSkyz

» Rare weather event reveals 5000 year old hidden artifacts!
Yesterday at 9:46 am by PurpleSkyz

» Another Actor Calls Out Satanic Hollywood Pedophiles
Yesterday at 9:43 am by PurpleSkyz

» You Will Question Everything After Watching This!
Yesterday at 9:38 am by PurpleSkyz

» The Flower Power Revolution - A Lesson In Naivety That Must Be Learned Today - David Icke
Yesterday at 9:34 am by PurpleSkyz

» Eyes wide Open - Pet Goat - The Last Piece of the Puzzle !?
Yesterday at 9:02 am by PurpleSkyz

» Ken O'Keefe - Trump, Free Expression, Red Pill Challenges Part 1
Yesterday at 8:58 am by PurpleSkyz

» Mozilla / Firefox goes all in for EVIL… pushes corporate news collusion to silence independent media
Thu Aug 16, 2018 6:55 pm by PurpleSkyz


CLICK THE SUBSCRIBE BUTTON BELOW TO RECEIVE OUR DAILY NEWSLETTER

A 2ND EMAIL COMPLETES THE ACTIVATION PROCESS




CLICK THE PURPLE BUTTON TO VIEW OUR LATEST POSTS



You are not connected. Please login or register

Out Of Mind » THE INSANITY OF REALITY » SCIENCE » Researchers create first stem cells using CRISPR genome activation

Researchers create first stem cells using CRISPR genome activation

Go down  Message [Page 1 of 1]

PurpleSkyz

avatar
Admin
Researchers create first stem cells using CRISPR genome activation

By
zIKY
-
January 18, 2018


Credit: CC0 Public Domain
 
by Gladstone Institutes
In a scientific first, researchers at the Gladstone Institutes turned skin cells from mice into stem cells by activating a specific gene in the cells using CRISPR technology. The innovative approach offers a potentially simpler technique to produce the valuable cell type and provides important insights into the cellular reprogramming process.
“This is a new way to make induced pluripotent stem cells that is fundamentally different from how they’ve been created before,” said author Sheng Ding, PhD, a senior investigator at Gladstone. “At the beginning of the study, we didn’t think this would work, but we wanted to at least try to answer the question: can you reprogram a cell just by unlocking a specific location of the genome? And the answer is yes.”
Pluripotent stem cells can be turned into virtually any cell type in the body. As a result, they are a key therapeutic resource for currently incurable conditions, such as heart failure, Parkinson’s disease, and blindness. They also provide excellent models to study diseases and important tools to test new drugs in human cells.
In 2006, Gladstone Senior Investigator Shinya Yamanaka, MD, PhD, discovered he could make stem cells—dubbed induced pluripotent stem cells (iPSCs)—by treating ordinary skin cells with four key proteins. These proteins, called transcription factors, work by changing which genes are expressed in the cell, turning off genes associated with skin cells and turning on genes associated with stem cells.
Building on this work, Ding and others previously created iPSCs not with transcription factors, but by adding a cocktail of chemicals to the cells. The latest study, published in Cell Stem Cell, offers a third way to turn skin cells into stem cells by directly manipulating the cells’ genome using CRISPR gene regulation techniques.
“Having different options to make iPSCs will be useful when scientists encounter challenges or difficulties with one approach,” said Ding, who is also a professor of pharmaceutical chemistry at the University of California, San Francisco. “Our approach could lead to a simpler method of creating iPSCs or could be used to directly reprogram skin cells into other cell types, such as heart cells or brain cells.”
CRISPR is a powerful tool that can precisely manipulate the genome by targeting a unique sequence of DNA. That sequence of DNA is then either permanently deleted or replaced, or it is temporarily turned on or off.
Ding’s team targeted two genes that are only expressed in stem cells and known to be integral to pluripotency: Sox2 and Oct4. Like transcription factors, these genes turn on other stem cell genes and turn off those associated with different cell types.
The researchers discovered that with CRISPR, they could activate either Sox2 or Oct4 to reprogram cells. In fact, they showed that targeting a single location on the genome was enough to trigger the natural chain reaction that led to reprogramming the cell into an iPSC.
For comparison, four transcription factors are typically used to create iPSCs using the original method. What’s more, one transcription factor typically targets thousands of genomic locations in the cell and changes gene expression at each location.
“The fact that modulating one site is sufficient is very surprising,” said Ding. “Now, we want to understand how this whole process spreads from a single location to the entire genome.”
 Explore further: Finding a key to unlock blocked differentiation in microRNA-deficient embryonic stem cells
More information: CRISPR-Based Chromatin Remodeling of the Endogenous Oct4 or Sox2 Locus Enables Reprogramming to Pluripotency, Cell Stem Cell (2018). dx.doi.org/10.1016/j.stem.2017.12.001 , www.cell.com/cell-stem-cell/fu … 1934-5909(17)30501-5

Journal reference: Cell Stem Cell  Provided by: Gladstone Institutes

Source: https://phys.org

Thanks to zIKY at: http://awakeandaware.ca



  

Back to top  Message [Page 1 of 1]

Permissions in this forum:
You cannot reply to topics in this forum