Latest topics
» Should we draw a conclusion ?
Today at 3:28 am by RamblerNash

Today at 3:07 am by RamblerNash

» Delusion Land: Anna Von Reitz & The Arrest Warrant - UPDATE: Owner and Operator of “Virgo Triad” YouTube Channel accused of attempted murder! WTF?
Today at 12:33 am by PurpleSkyz

» Leaked Image of Barack Obama Dressed as Satan Goes Viral??
Today at 12:01 am by PurpleSkyz

Yesterday at 8:15 pm by RamblerNash

» Jerzy/ZAP And The Saga Concerning "Mary" - FOUND!
Yesterday at 7:13 pm by RamblerNash

» Emotional Empathy, Emotional Intelligence and Psychopathy: What’s the Link?
Yesterday at 3:25 pm by PurpleSkyz

» How to Stop Worrying About the Future and Start Living Your Life Now
Yesterday at 3:23 pm by PurpleSkyz

» Morgue - Confederate Jesus Will Save You
Yesterday at 3:20 pm by PurpleSkyz

» CROP CIRCLE 2018 - Winterbourne Stoke Down, Stonehenge, Wiltshire. Reported 17th June
Yesterday at 3:14 pm by PurpleSkyz

» Hendricus Loos and Hulu
Yesterday at 2:23 pm by MartyM

» The Template Excerpt from The Ceremony of Sacred Breath. Code 3
Yesterday at 12:04 pm by Consciousness Of Economic

» The Template - The Ceremony of Synthesis - Code 2
Yesterday at 12:00 pm by Consciousness Of Economic

» The Template - The Ceremony of Original Innocence - Code 1
Yesterday at 11:52 am by Consciousness Of Economic

» UDEMY Online college Bridgette Lyn Dolgoff is getting her Courses up!
Yesterday at 10:34 am by Consciousness Of Economic

» How to Read Your Dreams (Part 1) – A Comprehensive Guide to Dream Interpretation - Know Your Dreams, Know Yourself
Yesterday at 9:56 am by PurpleSkyz

» Media Blackout On Navy Cyber Attack
Yesterday at 9:42 am by PurpleSkyz

» Have You Started Seeing 11:11 Everywhere?
Yesterday at 9:21 am by PurpleSkyz

» Forbes Says Self-Reliant Homesteaders Are “Delusional” and “Mooching” Off “Civil Society”
Yesterday at 9:20 am by PurpleSkyz

» Colloidal Silver’s Antimicrobial Properties And Safe Usage Suggestions
Yesterday at 9:17 am by PurpleSkyz

» Alan Watts - What if when we die we wake up?
Yesterday at 9:14 am by PurpleSkyz

» Tonight The Crescent Moon Meets Regulus, The Brightest Star In Leo
Yesterday at 9:05 am by PurpleSkyz

» Happy Fathers Day
Yesterday at 8:53 am by PurpleSkyz

» UFO News ~ Ufo Activity near the Sun plus MORE
Yesterday at 8:50 am by PurpleSkyz

» Side-Splitting Rebuke of the IG Report
Yesterday at 8:47 am by PurpleSkyz

Yesterday at 8:37 am by PurpleSkyz

» It's A Bird, It's A Fish!!! It's BOTH!!! New Creature Discovered???
Yesterday at 8:31 am by PurpleSkyz

» How to Free Your Mind from Mainstream Media Brainwashing
Yesterday at 8:27 am by PurpleSkyz

» Bilderberg and the Digital New World Order
Yesterday at 8:23 am by PurpleSkyz

» The Movement Has Started, The Clock Is Ticking Down, Tick Tock
Yesterday at 8:19 am by PurpleSkyz

» Temporal Bubbles Within Bubbles the Backbone of The Soul Stealing Grid
Yesterday at 8:17 am by PurpleSkyz

Yesterday at 12:07 am by MartyM

» Technology: Voice of God, OIW, Forced Dreams & Cybernetic Hive Mind
Sat Jun 16, 2018 11:56 pm by MartyM

You are not connected. Please login or register

Out Of Mind » THE INSANITY OF REALITY » SCIENCE » Researchers Find New DNA Structure in Living Human Cells

Researchers Find New DNA Structure in Living Human Cells

Go down  Message [Page 1 of 1]


Researchers Find New DNA Structure in Living Human Cells

April 29, 2018

A twisted ‘knot’ of DNA, the i-motif has never before been directly seen inside living cells. Image credit: Zeraati et al, doi: 10.1038/s41557-018-0046-3.
A team of scientists from the Garvan Institute of Medical Research and the Universities of New South Wales and Sydney has identified a new DNA structure — called the intercalated motif (i-motif) — inside living human cells.
Deep inside the cells in our body lies our DNA. The information in the DNA code — all 6 billion A, C, G and T letters — provides precise instructions for how our bodies are built, and how they work.
The iconic ‘double helix’ shape of DNA has captured the public imagination since 1953, when James Watson and Francis Crick famously uncovered the structure of DNA.
However, it’s now known that short stretches of DNA can exist in other shapes, in the laboratory at least — and scientists suspect that these different shapes might play an important role in how and when the DNA code is ‘read.’
“When most of us think of DNA, we think of the double helix. This research reminds us that totally different DNA structures exist — and could well be important for our cells,” said co-lead author Dr. Daniel Christ, from the Kinghorn Centre for Clinical Genomics at the Garvan Institute of Medical Research and St Vincent’s Clinical School at the University of New South Wales.
“The i-motif is a four-stranded ‘knot’ of DNA,” added co-lead author Dr. Marcel Dinger, also from the Garvan Institute of Medical Research and the University of New South Wales.
“In the knot structure, C letters on the same strand of DNA bind to each other — so this is very different from a double helix, where ‘letters’ on opposite strands recognize each other, and where Cs bind to Gs [guanines].”
Although researchers have seen the i-motif before and have studied it in detail, it has only been witnessed in vitro — that is, under artificial conditions in the laboratory, and not inside cells. In fact, they have debated whether i-motif DNA structures would exist at all inside living things — a question that is resolved by the new findings.
To detect the i-motifs inside cells, Dr. Christ, Dr. Dinger and their colleagues developed a precise new tool — a fragment of an antibody molecule — that could specifically recognize and attach to i-motifs with a very high affinity.
Until now, the lack of an antibody that is specific for i-motifs has severely hampered the understanding of their role.
Crucially, the antibody fragment didn’t detect DNA in helical form, nor did it recognize ‘G-quadruplex structures’ (a structurally similar four-stranded DNA arrangement).
With the new tool, the team uncovered the location of ‘i-motifs’ in a range of human cell lines.
Using fluorescence techniques to pinpoint where the i-motifs were located, the study authors identified numerous spots of green within the nucleus, which indicate the position of i-motifs.
The scientists showed that i-motifs mostly form at a particular point in the cell’s ‘life cycle’ — the late G1 phase, when DNA is being actively ‘read.’
They also showed that i-motifs appear in some promoter regions — areas of DNA that control whether genes are switched on or off — and in telomeres, ‘end sections’ of chromosomes that are important in the aging process.
“We think the coming and going of the i-motifs is a clue to what they do. It seems likely that they are there to help switch genes on or off, and to affect whether a gene is actively read or not,” said study first author Dr. Mahdi Zeraati, also from the Garvan Institute of Medical Research and the University of New South Wales.
“We also think the transient nature of the i-motifs explains why they have been so very difficult to track down in cells until now,” Dr. Christ added.
“It’s exciting to uncover a whole new form of DNA in cells — and these findings will set the stage for a whole new push to understand what this new DNA shape is really for, and whether it will impact on health and disease,” Dr. Dinger said.
The team’s results appear in the journal Nature Chemistry.
Mahdi Zeraati et al. I-motif DNA structures are formed in the nuclei of human cells. Nature Chemistry, published online April 23, 2018; doi: 10.1038/s41557-018-0046-3
Wake The Sheeple!

Thanks to zIKY at:


Back to top  Message [Page 1 of 1]

Permissions in this forum:
You cannot reply to topics in this forum