OUT OF MIND
Would you like to react to this message? Create an account in a few clicks or log in to continue.
Latest topics
» HIGH CRIMES: ‘Dangerous Alliance’, Who is Lynn Forester de Rothschild?
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:17 pm by PurpleSkyz

» Establishment Panics But Will Maxwell Walk?
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:09 pm by PurpleSkyz

» Federal Court Blocks Joe Biden’s Vaccine Mandate For Healthcare Workers in 10 States
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:00 pm by PurpleSkyz

» Scientists Confirm Earth Now Has a Second Moon: Here’s What We Know So Far
Researchers create first stem cells using CRISPR genome activation EmptyToday at 11:56 am by PurpleSkyz

» COVID Revealed Airs Tonight. Sign Up here...
Researchers create first stem cells using CRISPR genome activation EmptyToday at 11:42 am by PurpleSkyz

» Way to Go Home Depot Employees!
Researchers create first stem cells using CRISPR genome activation EmptyToday at 11:37 am by PurpleSkyz

» Misinformation And The Disappeared Truth
Researchers create first stem cells using CRISPR genome activation EmptyToday at 11:26 am by PurpleSkyz

» MURDER! Just Hours After Publishing the Secret of the Vaxx, Dr Noack is DEAD
Researchers create first stem cells using CRISPR genome activation EmptyToday at 11:13 am by PurpleSkyz

» Ghislaine Maxwell’s Trial Opens, With Epstein at Its Center
Researchers create first stem cells using CRISPR genome activation EmptyToday at 1:03 am by PurpleSkyz

» Solving the Riddle of Byram Bridle
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:58 am by PurpleSkyz

» No Vax, No Food/Fuel in India – Food Withheld to Force Vaccinations
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:56 am by PurpleSkyz

»  Former Pfizer Employee Says COVID-19 Vaccine Causes Recipients to Become More Susceptible to the Virus
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:53 am by PurpleSkyz

» Supply Chains and the Shortage Economy
Researchers create first stem cells using CRISPR genome activation EmptyToday at 12:48 am by PurpleSkyz

» Ultra-Fast Comet Leonard is Coming - Now's the Time to Start Tracking the Comet at Dawn
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:56 pm by PurpleSkyz

» Could we detect alien probes that are inside our own solar system ?
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:54 pm by PurpleSkyz

» James Comey’s daughter is a lead prosecutor in Ghislaine Maxwell’s child sex trafficking case. Here’s what we know about her
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:49 pm by PurpleSkyz

» ‘Sky Gods’, Credo Mutwa & The Alien Agenda
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:45 pm by PurpleSkyz

» USGS: Magnitude-7.5 earthquake strikes northern Peru
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:35 pm by PurpleSkyz

» Ten OMICRON “variant” predictions for 2022 and beyond
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:11 pm by PurpleSkyz

» Ancient Secret Societies, UFOs, and the New World Order
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:06 pm by PurpleSkyz

» The Van Allen Belts
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 1:02 pm by PurpleSkyz

» Report: World’s First Vaccine Murder Case Against Bill Gates Filed In India’s High Court
Researchers create first stem cells using CRISPR genome activation EmptyYesterday at 12:55 pm by PurpleSkyz





Holistic Alternative
Health Care
by Bridgette Lyn Dolgoff

CONTACT HERE TO SCHEDULE ONLINE SESSIONS
www.https://coe-llc.com

NEW ETSY SHOP FEATURING HERBAL SALVES

NOW MAKING A MEDICATED OIL FOR PEOPLE WHO HAVE PCR DAMAGE
www.https://coe-llc.com










Sway on Out Of Mind:
Sway's Website:
josuemercadoproductions.tk
Buy Me A Coffee:
PayPal:www.paypal.me/swaymerk CashApp:

You are not connected. Please login or register

OUT OF MIND » CHANGING ENVIRONMENT & NATURE » SCIENCE & TECHNOLOGY » Researchers create first stem cells using CRISPR genome activation

Researchers create first stem cells using CRISPR genome activation

Go down  Message [Page 1 of 1]

PurpleSkyz

PurpleSkyz
Admin

Researchers create first stem cells using CRISPR genome activation

By
zIKY
-
January 18, 2018

Researchers create first stem cells using CRISPR genome activation Mcvbvndu-696x418
Credit: CC0 Public Domain
 
by Gladstone Institutes
In a scientific first, researchers at the Gladstone Institutes turned skin cells from mice into stem cells by activating a specific gene in the cells using CRISPR technology. The innovative approach offers a potentially simpler technique to produce the valuable cell type and provides important insights into the cellular reprogramming process.
“This is a new way to make induced pluripotent stem cells that is fundamentally different from how they’ve been created before,” said author Sheng Ding, PhD, a senior investigator at Gladstone. “At the beginning of the study, we didn’t think this would work, but we wanted to at least try to answer the question: can you reprogram a cell just by unlocking a specific location of the genome? And the answer is yes.”
Pluripotent stem cells can be turned into virtually any cell type in the body. As a result, they are a key therapeutic resource for currently incurable conditions, such as heart failure, Parkinson’s disease, and blindness. They also provide excellent models to study diseases and important tools to test new drugs in human cells.
In 2006, Gladstone Senior Investigator Shinya Yamanaka, MD, PhD, discovered he could make stem cells—dubbed induced pluripotent stem cells (iPSCs)—by treating ordinary skin cells with four key proteins. These proteins, called transcription factors, work by changing which genes are expressed in the cell, turning off genes associated with skin cells and turning on genes associated with stem cells.
Building on this work, Ding and others previously created iPSCs not with transcription factors, but by adding a cocktail of chemicals to the cells. The latest study, published in Cell Stem Cell, offers a third way to turn skin cells into stem cells by directly manipulating the cells’ genome using CRISPR gene regulation techniques.
“Having different options to make iPSCs will be useful when scientists encounter challenges or difficulties with one approach,” said Ding, who is also a professor of pharmaceutical chemistry at the University of California, San Francisco. “Our approach could lead to a simpler method of creating iPSCs or could be used to directly reprogram skin cells into other cell types, such as heart cells or brain cells.”
CRISPR is a powerful tool that can precisely manipulate the genome by targeting a unique sequence of DNA. That sequence of DNA is then either permanently deleted or replaced, or it is temporarily turned on or off.
Ding’s team targeted two genes that are only expressed in stem cells and known to be integral to pluripotency: Sox2 and Oct4. Like transcription factors, these genes turn on other stem cell genes and turn off those associated with different cell types.
The researchers discovered that with CRISPR, they could activate either Sox2 or Oct4 to reprogram cells. In fact, they showed that targeting a single location on the genome was enough to trigger the natural chain reaction that led to reprogramming the cell into an iPSC.
For comparison, four transcription factors are typically used to create iPSCs using the original method. What’s more, one transcription factor typically targets thousands of genomic locations in the cell and changes gene expression at each location.
“The fact that modulating one site is sufficient is very surprising,” said Ding. “Now, we want to understand how this whole process spreads from a single location to the entire genome.”
Researchers create first stem cells using CRISPR genome activation 1x1  Explore further: Finding a key to unlock blocked differentiation in microRNA-deficient embryonic stem cells
More information: CRISPR-Based Chromatin Remodeling of the Endogenous Oct4 or Sox2 Locus Enables Reprogramming to Pluripotency, Cell Stem Cell (2018). dx.doi.org/10.1016/j.stem.2017.12.001 , www.cell.com/cell-stem-cell/fu … 1934-5909(17)30501-5

Journal reference: Cell Stem Cell  Researchers create first stem cells using CRISPR genome activation Img-dot Provided by: Gladstone Institutes

Source: https://phys.org

Thanks to zIKY at: http://awakeandaware.ca



  

Back to top  Message [Page 1 of 1]

Permissions in this forum:
You cannot reply to topics in this forum